Journal: The Journal of Physiological Sciences : JPS

Article Title: Exercise preconditioning-induced late phase of cardioprotection against exhaustive exercise: possible role of protein kinase C delta

doi: 10.1007/s12576-014-0323-x

Figure Lengend Snippet: Alteration of PKCδ expression in the late cardiac effect of EP. a Immunohistochemistry staining of PKCδ in cardiomyocytes. PKCδ demonstrated diffuse cytoplasmic staining in cardiomyocytes in all the groups (original magnification, ×400). C–N demonstrated the negative result of none treated with PKCδ primary antibody in control group. b Semi-quantitative analysis of PKCδ immunostaining. The positive area and IOD of the immunostaining in group EE and group LEP were significantly higher than those in group C (P < 0.05). Compared with group LEP + EE, the two values in group CHE + LEP + EE significantly increased (P < 0.05). c Myocardial PKCδ levels determined by western blotting. Consistent with the results of immunostaining, total PKCδ levels in group EE and group LEP were also significantly higher than those in group C (P < 0.05). Compared with group LEP + EE, PKCδ levels in group CHE + LEP + EE significantly increased (P < 0.05). Values are mean ± SD, significant differences (P < 0.05) are indicated as follows: from group C (*), from group LEP + EE (&)

Article Snippet: In situ hybridization In situ hybridization was performed according to previously described approaches [ 23 – 25 ], and an in situ hybridization detection kit for PKCδ mRNA (Boster Bio-engineering, Wuhan, China) was used.

Techniques: Expressing, Immunohistochemistry, Staining, Control, Immunostaining, Western Blot

Journal: The Journal of Physiological Sciences : JPS

Article Title: Exercise preconditioning-induced late phase of cardioprotection against exhaustive exercise: possible role of protein kinase C delta

doi: 10.1007/s12576-014-0323-x

Figure Lengend Snippet: Alteration of p-PKCδThr507 expression in the late cardiac effect of EP. a Immunohistochemistry staining of PKCδ in cardiomyocytes. p-PKCδThr507 demonstrated a granular, scattered cytoplasmic staining pattern in group C and group EE while the pattern changed in group LEP such that p-PKCδThr507 was preferentially localized to intercalated disks (arrow), but not in group LEP + EE and group CHE + LEP + EE. C–N demonstrated the negative result of none treated with p-PKCδThr507 primary antibody in the control group, original magnification, ×1,000. b Semiquantative analysis of p-PKCδThr507 immunostaining. The positive area and IOD of the immunostaining in group LEP + EE were significantly lower than those in group EE (P < 0.05). Compared with group LEP + EE, the two values in group CHE + LEP + EE were increased but not significantly (P < 0.05). c Myocardial p-PKCδThr507 levels determined by western blotting. Consistent with the results of immunostaining, total p-PKCδThr507 levels in group EE were significantly higher than those in group C (P < 0.05). However, compared with group EE, total p-PKCδThr507 levels in group LEP + EE were significantly decreased (P < 0.05). Compared with group LEP + EE, p-PKCδThr507 levels in group CHE + LEP + EE were significantly increased. Values are mean ± SD, significant differences (P < 0.05) are indicated as follows: from group C (*), from group EE (#), and from group LEP + EE (&)

Article Snippet: In situ hybridization In situ hybridization was performed according to previously described approaches [ 23 – 25 ], and an in situ hybridization detection kit for PKCδ mRNA (Boster Bio-engineering, Wuhan, China) was used.

Techniques: Expressing, Immunohistochemistry, Staining, Control, Immunostaining, Western Blot

Journal: The Journal of Physiological Sciences : JPS

Article Title: Exercise preconditioning-induced late phase of cardioprotection against exhaustive exercise: possible role of protein kinase C delta

doi: 10.1007/s12576-014-0323-x

Figure Lengend Snippet: Alteration of PKCδ mRNA expression in the late cardiac effect of EP. a Distribution of PKCδ mRNA in cardiomyocytes by in situ hybridization. The positive signal of PKCδ mRNA demonstrated a granular, diffuse cytoplasmic distribution pattern in cardiomyocytes. The intensity of the hybridization signal was markedly strong in group LEP. C–N, the negative result of none treated with PKCδ mRNA probes in the control group. b Semiquantative analysis of the positive signal of PKCδ mRNA. The area and IOD of the positive signal of PKCδ mRNA in group LEP were significantly higher than those in group C. The c PKCδ mRNA levels determined by quantitative real-time PCR. PKCδ mRNA levels in group LEP were significantly higher than those in group C (P < 0.05). Values are mean ± SD, significant differences (P < 0.05) are indicated as follows: from group C (*), from group EE (#)

Article Snippet: In situ hybridization In situ hybridization was performed according to previously described approaches [ 23 – 25 ], and an in situ hybridization detection kit for PKCδ mRNA (Boster Bio-engineering, Wuhan, China) was used.

Techniques: Expressing, In Situ Hybridization, Hybridization, Control, Real-time Polymerase Chain Reaction